Jacobs thumbed his nose at the 16-year-old age limit suggested by the Council of Fashion Designers of American (CFDA) by sending 14-year-old Ford models Thairine Garcia and Ondria Hardin down the runway.
The move has outraged many in the fashion world who believe Ford, a member of the board of CFDA, should show greater respect for the new rules.
Jacobs use of young models is a blow for the CFDA which has only just set the new guidelines, aimed at keeping models healthy.
The president of the CFDA, designer Diane von Furstenberg, wrote in the CFDA guidelines that models were under increasing pressure to be "thinner and younger".
"The industry's hiring of prepubescent-appearing teenage girls as models of adult clothing sets an unrealistic standard; hips and breasts, the curves that define the female figure, are absent," she wrote.
"Some models have difficulty maintaining the body ideal as they move into adulthood and run the risk of engaging in unhealthy eating behaviors that lead to eating disorders."
"In January 2007, the CFDA formed a health initiative to address what has become a global fashion issue: the overwhelming concern about whether some models are unhealthily thin, and whether or not to impose restrictions in such cases.
"Designers share a responsibility to protect women, and very young girls in particular, within the business, sending the message that beauty is health."
Jacobs, however, was unrepentant, telling The New York Times: “I do the show the way I think it should be, and not the way somebody tells me it should be.”
The designer compared the models to child actors saying: “If their parents are willing to let them do a show, I don’t see any reason that it should be me who tells them that they can’t.”